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Antioxidant capacity and structural changes of human serum albumin from patients in advanced stages of diabetic nephropathy and the effect of the dialysis

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Abstract

Changes in the antioxidant capacity of albumin and alterations of the albumin structural conformation were examined in patients in advanced stages of diabetes nephropathy. Human serum albumin was purified from diabetic patients in pre-dialysis (glomerular filtration rate [GFR] between 15 and 29 ml min−1 1.73 m−2) and those in dialysis (GFR ≤ 15 ml min−1 1.73 m−2) and then compared with albumin from patients with a normal GFR (>90 ml min−1 m−2). We evaluated the antioxidant capacity of albumin using an enhanced chemiluminescence-based assay and thiol group content, and the structural changes were evaluated by circular dichroism and fluorescence spectroscopy. The antioxidant capacity and thiol content of albumin from patients in advanced stages of diabetic nephropathy were markedly reduced. The circular dichroism spectra showed a mean albumin α-helix content reduction from 44 to 37 % and from 44 to 30 % between the control group and pre-dialysis and dialysis patients, respectively. Additionally, the fluorescence intensity was reduced by 4.2 and 13 % for the groups 4 and 5, respectively, in relation with the control. These data provide evidence for the partial denaturation of albumin and exacerbated oxidative stress among patients in advanced stages of diabetes nephropathy before and even after dialysis.

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Acknowledgments

This study was supported by the Fondo de Investigación en Salud FIS/IMSS/PROT/G10/854 and FIS/IMSS/PROT/896/Consejo Nacional de Ciencia y Tecnología (CONACYT) SALUD-2010-01-141937.

Conflict of Interest

The authors have no conflicts of interest associated with the content of this manuscript.

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Authors and Affiliations

  1. Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), Camino de la Arboleda 300, Ex Hacienda de San José La Huerta., Morelia, Michoacán, C.P. 58341, Mexico

    Marisol Rosas-Díaz & Rafael Medina-Navarro

  2. Department of Physical Chemistry, Univesidad Autónoma Metropolitana de Iztapalapa (UAM-Iztapalapa), Mexico, DF, Mexico

    Menandro Camarillo-Cadena & Andrés Hernández-Arana

  3. Environmental Cytopathology Laboratory, ENCB-IPN, Mexico, DF, Mexico

    Eva Ramón-Gallegos

Authors
  1. Marisol Rosas-Díaz
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  2. Menandro Camarillo-Cadena
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  3. Andrés Hernández-Arana
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  4. Eva Ramón-Gallegos
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  5. Rafael Medina-Navarro
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Correspondence to Rafael Medina-Navarro.

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Rosas-Díaz, M., Camarillo-Cadena, M., Hernández-Arana, A. et al. Antioxidant capacity and structural changes of human serum albumin from patients in advanced stages of diabetic nephropathy and the effect of the dialysis. Mol Cell Biochem 404, 193–201 (2015). https://doi.org/10.1007/s11010-015-2378-2

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  • DOI: https://doi.org/10.1007/s11010-015-2378-2

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