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A study on the interaction between 5-Methyluridine and human serum albumin using fluorescence quenching method and molecular modeling

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Abstract

This work was designed to study the interaction between 5-Methyluridine and human serum albumin (HSA) under simulative physiological conditions using fluorescence spectroscopy in combination with molecular modeling technique for the first time. Static quenching was suggested by the fluorescence measurement. The binding constants (K) were calculated according to the relevant fluorescence data at different conditions including temperature. Thermodynamic parameter, different conditions including temperature to determine enthalpy change and entropy change, indicating the hydrophobic force played a major role in the binding interaction between 5-Methyluridine and HSA. The experimental result was in correspondence with molecular modeling theory.

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Acknowledgments

This work was sponsored by the Nature Science Foundation of China (Nos. 20673034, 20772024), the special foundation of Chinese Ministry of Education in 2006, for Dr universities teaching and scientific research (No.20060476001) and Department of Education of Henan Province (No.2006150012).

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Authors and Affiliations

  1. School of Chemistry and Environmental Science, Key Laboratory for Yellow River and Huai he River Water Environment and Pollution Control Ministry of Education, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Feng-Ling Cui, Ying-Hua Yan, Qiang-Zhai Zhang & Gui-Rong Qu

  2. Department of Chemistry, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Juan Du & Xiao-Jun Yao

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  1. Feng-Ling Cui
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  2. Ying-Hua Yan
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Correspondence to Feng-Ling Cui.

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Cui, FL., Yan, YH., Zhang, QZ. et al. A study on the interaction between 5-Methyluridine and human serum albumin using fluorescence quenching method and molecular modeling. J Mol Model 16, 255–262 (2010). https://doi.org/10.1007/s00894-009-0548-4

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  • DOI: https://doi.org/10.1007/s00894-009-0548-4

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