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. 2015 Nov 6;5:16148. doi: 10.1038/srep16148

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(a) Flow cytometry DC gating and SCFA effect. The figure is representative of the gating strategy for DC for HLA-DR, CD83, and CD86 markers for both monocyte-derived im-DC and m-DC. (b) A representative example of SCFA effect on im-DC and m-DC expression of CD86 and CD83 markers by flow cytometry. (c) Effect of SCFA on DC maturation markers. Numbers calculated represented by flow cytometry indicate the mean fluorescence intensity (MFI) of each sample. Shown are the averages ± standard deviations (SD) (n = 3); Mann-Whitney U t-test, *P < 0.05.