Figure 8. PDHK is selectively required for Th17, but not Treg, expansion and function in vivo.
(A–C) Rag1–/– mice were injected with naive Teffs (CD4+CD25–CD45RBhi), and colitis was induced. Mice were given 2 g/l of DCA (n = 10) or vehicle (n = 10) in the drinking water for the duration of the experiment. (A) The number of CD4+ T cells or (B and C) the percentages of IFN-γ– and IL-17–producing cells in the spleen and mesenteric lymph nodes were determined using flow cytometry. (D–H) EAE was induced in wild-type mice with or without DCA treatment (10 mice per group). (D) A time course of clinical scores is shown. (E and F) The percentages of (E) CD4+FoxP3+ and (F) CD4+IL-17+ T cells in the draining lymph nodes on day 9 were determined using flow cytometry. (G) H&E and (H) LFB staining were performed on spinal cord sections from mice with active disease. Data are shown as mean ± SD (A–C, E, and F), and data are representative of 3 independent experiments. *P < 0.05.