Fig. 1. Luteolin inhibits uric acid-stimulated NO formation and iNOS expression.
A: After Min6 cells were treated with or without 10 μmol/L luteolin in the presence/absence of 5 mg/dL uric acid for 24 hours, NO formation in medium was measured. n = 4. B: After Min6 cells were treated as described above, the expression of iNOS mRNA of cells was determined by real time PCR. n = 4. C: The expression level of iNOS protein of differently treated Min6 cells were measured by Western blotting assays. D: All blots were repeated for 3 times. The densitometric analysis of protein bands were performed with Quality One software. * P < 0.05 indicates significant difference compared to the uric acid-treated alone group (A, P = 0.008 and 0.011, respectively; B, P = 0.003 and 0.003; D, P < 0.001 and = 0.012, respectively). NO: nitric oxide; iNOS: inducible NO synthase