Figure 3.
Genistein (G)-induced β-cell proliferation is ER independent. A, INS1 cells were preincubated with ICI (I; 2 μm) or vehicle for 30 min, followed by addition of G (1 μm) or vehicle (C). B, INS1 cells were incubated with G (1 μm), 17β-estradiol (E1; 1 nm; E10; 10 nm) or vehicle (C) in RPMI 1640 containing 1 mm glucose. Twenty-four hours later, cell proliferation was measured with ELISA. Data obtained from five separate experiments in triplicate determinations each are expressed as mean ± se. *, P < 0.05 vs. vehicle-alone-treated cells.