Figure 1.
Genistein (Gen) stimulates proliferation of β-cells. A, INS1 cells were incubated with various concentrations of Gen or vehicle in RPMI 1640 medium for 24 h, followed by addition of BrdU (10 μm) for 4 h. Cell proliferation were determined by measuring DNA synthesis using BrdU ELISA kit. Data were expressed as mean ± se of observations from four to five separate experiments, each performed in triplicate sample. B, INS1 cells were incubated with Gen (1 μm) or vehicle [control (C)] for 3 h. The level of cyclin D1 in the treated cell extracts was measured by Western blot and normalized to β-actin content from the same sample. The bar graphs (mean ± se) represent four independent experiments. *, P < 0.05 vs. vehicle-alone-treated control.